Twentythree hydrocarbondegrading bacteria strains were isolated from gas. Due to the importance of these hydrocarbons, they have become an important source of energy, resulting in a large number of residues in the environment, causing many. Anaerobic degradation of monoaromatic hydrocarbons. Microbial degradation of hydrocarbons in the environment.
One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Pdf microbial degradation of petroleum hydrocarbon. Although bacterial anaerobic degradation of monoaromatic compounds has been characterized in depth, the degradation of polycyclic aromatic hydrocarbons pahs such as naphthalene has only started. Comparative studies on bacterial consortia for hydrocarbon. Therefore, study of microbial communities adapted to microaerob conditions is always required.
Microbial degradation of hydrocarbons in the marine environment, with a. The key differences between petroleum biodegradation in soil and aquatic ecosystems following an oil spill, discussed by bossert and bartha 29, are related to. Environmental studies on the microbial degradation of oil. Microbial degradation of hydrocarbons in the environment ncbi. Microbial degradation of petroleum hydrocarbons ncbi. Environmental degradation of petroleum hydrocarbons utrecht.
Recent studies in microbial degradation of petroleum. Anaerobic biodegradation of saturated and aromatic. Biodegradation of crude petroleum in an acid soil ph 4. Index terms microorganisms, hydrocarbon, bioremediation i. The alephatics the aromatics the asphaltenes phenols, fatty acids, ketones, esters, and porphyrins, and the resins pyridines, quinolines. Microbial degradation ofhydrocarbons in the environment. Biodegradation of petroleum hydrocarbons in soil by. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated.
Science institute, ahmedab ad, gujarat, india corresponding author. Stimulated anaerobic degradation of petroleum hydrocarbons. Comparative studies on bacterial consortia for hydrocarbon degradation. Details of enzymes involved in petroleum hydrocarbon pollutant degradation along with hydrocarbon pollutant degraded and microorganism for the same are depicted in table 2. Petrochemical contaminated site was found to be a rich source of microbial consortium degrading polycyclic aromatic hydrocarbons. The most common problems today are caused due to petroleum products, their use and their degradation. Anaerobic microbial degradation of polycyclic aromatic. Environmental studies on the microbial degradation of oil hydrocarbons and its application in lebanese oil polluted coastal and marine ecosystem darin maliji, zakia olama and hanafy holail biological and environmental science department, beirut arab university, beirut, lebanon corresponding author email. The rates of mineralization of the highermolecularweight aromatic hydrocarbons, such as naphthalene and phenanthrene, are related to aqueous solubilities rather than total substrate concentrations 5, 152, 153. Biodegradation of hydrocarbons in the environment microbial degradation of hydrocarbons may apply also to soil ecosystems. Anaerobic degradation of monoaromatic hydrocarbons springerlink. The composition and inherent biodegradability of the petroleum hydrocarbon pollutant is the first and foremost important consideration when the. Microbial degradation of petroleum biodegradation of hcs is a complex process depending on.
Bioremediation of polycyclic aromatic hydrocarbons in soil at former manufactured gas plant sites jeremy m. A number of limiting factors have been recognized to affect the biodegradation of petroleum hydrocarbons, many of which have been discussed by brusseau. Anaerobic biological degradation of aromatic hydrocarbons. Biodegradation of petroleum hydrocarbons in soil by microbial. Such degradation can occur aerobically, with oxygen, or anaerobically, with nitrate, ferric iron, sulfate or other electron acceptors. Nitrate did not affect the ph, whereas ammonium amendment led to progressive acidification, accompanied by an inhibition of the degradation of aromatic, particularly polycylic aromatic hydrocarbons pahs. The bacteria ability to degrade different hydrocarbons. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons e. This industry has immense importance in the growth of economy and manufacture of large varieties of chemicals. Novosphingobium pentaromativorans us61, a marine bacterium isolated from muddy sediments of ulsan bay, republic of korea, was previously shown to be capable of degrading multiple polycyclic aromatic hydrocarbons pahs.
Stimulated anaerobic degradation of petroleum hydrocarbons the aerobic degradation of petroleum hydrocarbons has been well demonstrated. The optimum ph for biodegradation of hydrocarbons is around 68 22. Biodegradation of hydrocarbons is a costeffective technique that is based on highly dispersed microbes in soil and water capable of biodegrading hydrocarbons. Anaerobic degradation of oil hydrocarbons by sulfate. Focus on the microbial response to the deepwater horizon oil spill. Due to the major oil due to the major oil spills which took place during last few decades, n eed for effective strategy to deal. Microbial polycyclic aromatic hydrocarbons degradation in soil. In this study we have investigated the ability of microorganisms present in the soil itself to degrade these hydrocarbons so that contaminated soils can be treated using microbial consortium. Depending on the chain length and type of petroleum hydrocarbon pollutants different enzymes are required to introduce oxygen in substrate to start biodegradation. In soil remediation, aerobic degradation is often used for degradation of aromatic hydrocarbons, such as benzene.
Introduction the biodegradation of petroleum and other hydrocarbons in the environment is a complex process, whos quantitative and qualitative aspects depend on the nature and amount of the oil or hydrocarbons present, the ambient and seasonal environmental. Aliphatic hydrocarbons become less water soluble with increasing chain length. Pah biodegradation, planomicrobium, rhodococcus, phenanthrene, pyrene. Microbial degradation of petrochemical wastepolycyclic. Two groups of pseudomonas species that assimilate components of common petroleum based accelerants have been found. The invention relates to a process for the anaerobic biodegradation of aromatic hydrocarbons, as well as to a specific mixture and its use for this degradation. Several bacteria are even known to feed exclusively on hydrocarbons 39. Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons. In order to gain insight into the characteristics of pahs degradation, a proteome analysis of n. Bacteria play an important role in the microbial degradation of oil, chlorinated hydrocarbons, fuel additives, and many other compounds. Petroleum hydrocarbons and chlorinated hydrocarbons.
We are committed to sharing findings related to covid19 as quickly and safely as possible. The mechanism of cyclohexane degradation is shown in fig. Degradation of hydrocarbons in spent engine oil contaminated soil by pseudomonas aeruginosa 612 degradation of different hydrocarbons prescott et al. Alkanes, a major fraction of crude oil, are saturated hydrocarbons and hence are chemically inert as nonpolar molecules. Salinity and pressure may also affect biodegradation rates in some aquatic environments, and moisture and ph may limit biodegradation in soils. Apr 23, 2014 degradation of hydrocarbons in hypersaline environments.
Richnowa, eva annweilerb, walter michaelisb, rainer u. Figure 3 microbial degradation of petroleum hydrocarbon. If exposed to the environment they can be hazardous to the surroundings as well. Effect of btex concentration and environment final report november 15, 2002 dsr contact person. Microbial in situ degradation of aromatic hydrocarbons in a contaminated aquifer monitored by carbon isotope fractionation hans h.
Microbial degradation of petroleum hydrocarbons in a polluted tropical stream sunday a. Assessing in situ degradation of petroleum hydrocarbons by indigenous. Bioremediation is a noninvasive and cost effective technique for the cleanup of these petroleum hydrocarbons. Biodegradation and bioremediation of hydrocarbons in extreme.
Maximum utilization of hydrocarbons from crude oil was in dicated by the total discoloration of dcpip. Anaerobic biodegradation of saturated and aromatic hydrocarbons. In addition, the initial enzymes for degradation of biphenyl have the ability to metabolize a wide variety of different polycyclic aromatic hydrocarbons. Introduction rates of biodegradation depend greatly on the composition,petroleum is a liquid mixture consisting of complex hydrocarbons. Indigenous microbial consortiums were identified and used for bioremediation of polycyclic aromatic hydrocarbons naphthalene and anthracene at the concentrations of 250, 500, and 750 ppm. Microbial in situ degradation of aromatic hydrocarbons in. Microbial degradation of hydrocarbons in the marine. Noincrease was observed at a level of 10%, and the rates declined at 15%. We isolated 14 different bacteria from various petroleum contaminates sites, using enrichment technique, and studied for utilization of various hydrocarbons.
Comparative studies on bacterial consortia for hydrocarbon degradation sunita j. However, a number of bacterial and fungal genera have been reported to degrade even high molecular weight alkanes in both aerobic and anaerobic conditions. Isolation and characterization of hydrocarbondegrading. The nature of this contamination is organic compounds in.
The use of thermophiles for biodegradation of hydrocarbons with low water solubility is of interest, as solubility and thus bioavailability, are enhanced at elevated temperatures. May 03, 2015 biodegradation of petroleum hydrocarbons by. For water treatment absorption and biodegradation are the most used methods. Meckenstockc adepartment of remediation research, ufzcentre for environmental research leipzighalle gmbh, permoserstrasse 15, d04318 leipzig, germany. Table 1 microbial degradation of petroleum hydrocarbon. The degradation of phenanthrene in liquid media was favorable at a range of ph values ph 6. For information about anaerobic degradation of alkenes and alkynes, the reader is referred to other overview articles 4,5.
Jan 21, 2004 in addition, it has also been recently shown that complete degradation of monoaromatic hydrocarbons can also be coupled to the respiration of oxyanions of chlorine such as perchlorate or chlorate, or to the reduction of the quinone moieties of humic substances. Degradation is an effective method for remediation of petrohydrocarbons and causes changes in nature and concentration of petrocompounds. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38. Efforts to overview the metabolism of hydrocarbons in microorganisms are confronted with the chemical diversity of such compounds and their reactivities, as well as with various microbial life styles. Anaerobic degradation of monoaromatic hydrocarbons anaerobic degradation of monoaromatic hydrocarbons chakraborty, r coates, j. Aromatic hydrocarbon degradation by sphingomonas yanoikuyae b1. It is now known that compounds such as benzene, toluene, ethylbenzene, and all three xylene isomers can be biodegraded in the absence of oxygen by a broad diversity of organisms. Microbial degradation of petroleum hydrocarbons sciencedirect.
Petroleum hydrocarbons and chlorinated hydrocarbons differ in. Petroleum is a complex mixture of different hydrocarbons including aliphatic linear or branched, cycloalkanes, mono and polyaromatics, asphaltenes and resins and majority of these compounds are stable, toxic, and carcinogenic philip et al. Many polycyclic aromatic hydrocarbons pahs are known to be toxic and carcinogenic for humans, and their contamination of soils and aquifers is of great environmental concern. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. Upon this background, pseudomonas aeruginosa strain known to have hydrocarbon degradative ability was developed and used. In subsurface ecosystems, the availability of oxygen is often restricted. The petrochemical industry is a hazardous group of industry generating hazardous waste containing organic and inorganic compounds. Upasani 2 1faculty, gujarat national law university gnlu, koba, gandhinagar, gujarat, india 2associate professor, department of microbiology, m. Biodegradation of polycyclic aromatic hydrocarbons by. Hydrocarbons with a chain length of c 12 and above are virtually water insoluble. Formation of aliphatic and aromatic hydrocarbons biological formation among hydrocarbons formed by living organisms, one may. Adaptation by prior exposure of microbial communities to hydrocarbons increases hydrocarbon degradation rates. An overview on microbial degradation of petroleum hydrocarbon. The complete degradation of pahs that originate from various sources require a community of microorganisms.
In oxidized state the i ndicator is blue and its reduced state is colourless. Due to the importance of these hydrocarbons, they have become an important source of energy, resulting in a large number of residues in the environment, causing many negative environmental impacts. Over the last two decades significant advances have been made in our understanding of the anaerobic biodegradability of monoaromatic hydrocarbons. Isolation and molecular identification of hydrocarbon. If you continue browsing the site, you agree to the use of cookies on this website. Bacterial degradation of the polycyclic aromatic hydrocarbon pah fraction of refinery effluent. Polycyclic aromatic hydrocarbons pahs is the common name for a class of several hundred different compounds, which typically contain two to seven condensed benzene rings. It is the main source of energy for industry and daily life. Thermophiles, predominantly bacilli, possess a substantial potential for the degradation of environmental pollutants, including all major classes. Microbial degradation of petroleum hydrocarbon contaminants. Biodegradation of petroleum hydrocarbons slideshare. Principles of microbial degradation of petroleum hydrocarbons. Hydrocarbons are degraded primarily by bacteria and fungi.
In spite of the present treatment process, the hazardous waste compounds are found. Anaerobic degradation of oil hydrocarbons by sulfatereducing. Biodegradation and bioremediation of hydrocarbons in. In general,alkyl side chains of cycloalkanes facilitate the degradation. This study was aimed at bacterial degradation of polycyclic aromatic hydrocarbons pah in untreated refinery effluent.
Degradation of polyaromatic hydrocarbons by mixed culture. Isolation and molecular identification of hydrocarbon degrading bacteria from oilcontaminated soil fatiha dilmi 1, 2, abdelwaheb chibani 1, khadidja senouci rezkallah 2 1laboratory of microbiology and plant science, department of biology, faculty of natural and life sciences, university of abdelhamidibn badis, mostaganem, algeria. Olatope 2 1 department of botany and microbiology, university of lagos, lagos, nigeria. Degradation of polyaromatic hydrocarbons by mixed culture isolated from oil contaminated soila bioprocess engineering study ruma roy, raja ray 1, ranjana chowdhury, pinaki bhattacharya chemical engineering department, jadavpur university, kolkata 700 032, india. Quite a lot of research has been carried on hydrocarbon degradation. Hence, studying the biological degradation or degradability of hydrocarbons means directing our research interest not only to an. Polycyclic aromatic hydrocarbons pahs are a class of toxic environmental pollutants that have accumulated in the environment due to a variety of anthropogenic activities.
The catabolic pathways for the degradation of both the monocyclic. Evidence for cooxidation of asphaltenes was provided by rontani et al. Dibble and bartha 51 reported increases in co2evolution overthe range of1. The biodegradation of hydrocarbons in terrestrial ecosystems may be restricted because of the water available for metabolism and growth of microbial. Dec 08, 2014 microbial degradation of petroleum biodegradation of hcs is a complex process depending on. Biodegradation of petroleum compound using the bacterium. Contaminated soil samples were collected from warri refinery jetty while water sample was. Common pathway for hydrocarbon degradation include initial degradation of alkane with oxidation of methyl group and results in formation of an alcohol, which is then dehydrogenated via aldehyde to corresponding carboxylic acid, which can then be metabolized by. The alephatics the aromatics the asphaltenes phenols, fatty acids, ketones, esters, and porphyrins, and the resins pyridines, quinolines, carbazoles, sulfoxides, and amides cont. Ex situ aerobic degradation is straightforward, however, the in situ transport properties of oxygen are poor due to low solubility and high reactivity. The occurrence of polycyclic aromatic hydrocarbons pah in refinery effluents is of great concern globally due to its persistence, recalcitrance and carcinogenicity.
520 1553 1204 710 1265 1125 340 952 436 524 775 1403 609 928 1446 274 494 272 410 1257 62 423 533 1195 197 1040 1094 1154 583 1105 49 341 904 1178 848 1079 1125